can anyone recommend an idt prime time qpcr control probe for

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Can anyone recommend an IDT Prime Time qPCR control probe …


Can anyone recommend an IDT Prime Time qPCR control probe for mouse-derived cells? I am performing qPCR on a mouse cell line, and I need a recommendation on a control. We typically use IDT Prime

PrimeTime qPCR Primer Assays – Integrated DNA Technologies


PrimeTime qPCR Primer Assays are ideal for use with SYBR Green, EvaGreen, and other intercalating dyes, where no probe is needed. PrimeTime qPCR Primer Assays are available in standard size, premixed, normalized to 5 nmol per primer, and shipped dried down. Primer sequences are provided upon order, and the assays ship in 2–3 business days.

PrimeTime qPCR Probes – Integrated DNA Technologies


PrimeTime Eco qPCR Probes. The PrimeTime Eco qPCR Probe is the ideal scale when you need to perform ~500 reactions for gene expression analysis or genotyping. The combination of medium scale and low cost is ideal for initial screening of large sample …

PrimeTime qPCR Probes | IDT


We now offer Affinity Plus qPCR Probes — a new locked nucleic acid modified qPCR probe. These affordable qPCR probes can increase Tm to provide greater sensitivity and mismatch discrimination. Find out more here.

Custom qPCR probes – Integrated DNA Technologies ǀ IDT


IDT qPCR probes are HPLC purified to ensure removal of free residual dye and truncated synthesis products that can contribute to high background signal. Good probe design must balance the need for high affinity with considerations for best signal generation and quenching. We have a variety of options to address even complex design requirements.

Expert help for optimizing gene expression | IDT


We can help you with that! Optimize your gene expression experiments with our dual-labeled PrimeTime qPCR probes. Successfully multiplex with reduced crosstalk and background signal, even with longer probes, when using ZEN™ or TAO™ Double-Quenched Probes. These 5′ nuclease probes are available with an assortment of reporter-dye …

Using qPCR assays in multiplex experiments


Jun 17, 2011 · The primer-to-probe ratios that work best will depend on your particular experiments, but a good starting point is to limit the primer-to-probe ratio for the highest expressing genes to 1:1 and increase the ratio for the lowest expressing genes to 4:1. IDT PrimeTime qPCR Assays allow you to select a premixed primer/probe ratio from 1:1 to 4:1 …

Can anyone recommend a reference gene for qRT-PCR for mRNA …


So to cover this issue I would recommend doing the QPCR two ways. 1. Input the same number of ug into your QPCR assay. 2. Input the same fraction of total RNA yield from each treatment into your …